
Fig. 7. Cell structure in an E2748 seed (160 mg seed in A-C and 260 mg seed in D-E), Toluidine Blue staining in A,D and transmission electron microscopy in B,C,E. (A) Contact zone of cotyledon with seed coat (arrowhead 1) and with the endosperm (arrowhead 2). (B) Ultrastructure of the cotyledon to seed coat contact zone. (C) Ultrastructure of the cotyledon with endosperm contact zone, note the irregularly thickened cell wall (arrowheads). (D) Contact zone of cotyledon with seed coat (260 mg seed). (E) Ultrastructure of the cotyledon to seed coat contact zone. Note the large differences in cell size of the outermost cells. ch, chloroplast; en, endosperm; er, endoplasmic reticulum; n, nucleus; v, vacuole. Scale bars, 40 µm (A,B,D,E), 5 µm (C).