Fig. 8. Cotyledon cell morphology and starch deposition in wild-type (A,C,E) and mutant embryos (B,D,F) at mid-cotyledon stage. The section through the abaxial region of a mutant embryo shows highly vacuolated cells (arrowhead) on the surface and in the outer tissue layers of the cotyledon, whereas cell sizes increase toward the interior (B). After iodine staining in wild-type cotyledons, starch grains are barely recognisable because of the low resolution (C). Starch deposition in the outer tissue layers of the mutant cotyledon (D). In the seed coats of both wild-type and mutant seeds starch accumulation is similar (C,D). Accumulation of starch grains during maturation coincides with the different cell size gradients in both wild-type (E) and mutant cotyledons (F). Scale bars: 125 µm (A,B); 450 µm (C,D); 200 µm (E,F).
