Fig. 9. Transcript levels of transport- and storage-associated genes in embryos (A,C,E,G,I,K) and seed coats (B,D,F,H,J,L) of E2748 and wild-type cotyledons during development. The experiment had been repeated twice with similar results. The figure shows representative results from a single experiment. Identical blots were hybridised by sequential rounds of northern hybridisations with probes detecting the following mRNAs: sucrose transporter (PsSUT1; A,B); potassium channel (PsKT1; C,D); H+-ATPase (PsATP1; E,F); sucrose synthase (PsSUS1; G,H); ADP glucose pyrophosphorylase large subunit (PsAGPL; I,J); plastidial phosphoglucomutase (PspPGM; K,L). The values on the x axis refer to seed fresh weight of wild-type seeds. Black symbols: mutant; white symbols: wild type.
