Fig. 4. spt6 and spt5 mRNA rescue the defects in pan and fogs30 mutants, respectively. Uninjected embryos (A,B,E,F,I,J,M,N) are compared with embryos that were injected with synthetic mRNA, either spt6 (C,D,G,H) or spt5 (K,L,O,P). All embryonic genotypes were confirmed following photography (see Materials and Methods). Genotypes are: wild-type embryos (A,C,E,G,I,K,M,O); pan mutants (B,D,F,H); and fogs30 mutants (J,L,N,P). (A-D,I-L) Lateral views of live embryos at 36 hpf, anterior towards the left. Injection of wild-type mRNA rescues pigmentation, tail length, ear formation and cardiac function in mutants, and does not affect wild-type siblings. Rescued embryos survive for 4-5 days post-fertilization. (E-H,M-P) Whole-mount in situ hybridization indicates expression of cmlc2 at 16.5-17.5 hpf; dorsal views of embryos, anterior towards the top. Injection of wild-type mRNA rescues the timely and robust expression of cmlc2 expression in mutants and does not affect expression in wild-type siblings.
