Fig. 5. spt6 and spt5 are expressed maternally and zygotically throughout the zebrafish embryo. Whole-mount in situ hybridization, comparing the expression patterns of spt6 (A-C) and spt5 (D-F). Wild-type embryos at 2.75 hpf (512-cell) (A) or 2.25 hpf (128-cell) (D) demonstrate that both spt6 (A) and spt5 (D) are expressed throughout the blastoderm before the initiation of zygotic transcription and are therefore maternally supplied. (A,D) Lateral views, with the animal pole at the top. Wild-type embryos at 16.5 hpf (B) or 10 hpf (E) demonstrate that spt6 (B) and spt5 (E) are expressed throughout the embryo after the initiation of zygotic transcription. Inset in E depicts a fog^s30 mutant embryo at 10 hpf. spt5 mRNA is barely detectable in fog^s30 mutants at this stage, in keeping with the deletion of spt5 in fog^s30 mutant genomic DNA. (B,E) Lateral views, anterior towards the top. Wild-type embryos at 24 hpf (C) or 26 hpf (F) demonstrate that spt6 (C) and spt5 (F) are still broadly expressed, with highest levels in the embryonic brain. (C,F) Lateral views, anterior towards the left.

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