Fig. 4. Generation and characterization of transgenic mice and ANSCs. (A) The structure of the transgene driving ectopic-heterochronic expression of Emx2 and the location of both oligos and probes employed for the analysis of transgene expression. These include the two riboprobes, tg-A and tg-B, used for the RNAse protection assay; the three primers, RT, PF and PR, used for the RT-PCR assay; the Emx2 DNA probe, SP, hybridized to RT-PCR products to confirm their specificity. (B,C) Expression of Emx2 in embryonic telencephalon of wild-type and transgenic mice, as measured by the RNAse protection assay. (D) Expression of the Emx2 transgene in the forebrain periventricular tissue dissected from adult wild-type and mutant mice belonging to one of the two lines used, as assayed by RT-PCR-Southern blotting assay. Similar results were obtained for the other transgenic line (not shown). (E) Expression of the Emx2 transgene in ANSCs isolated and propagated from the same tissues described in D is shown.
