Fig. 2. Expression analysis of Igf2 and H19 in Mnt. (A) Northern blot analysis of total RNA prepared from the different classes of E14 intercross embryo and placenta samples; Mnt^P n=4; Mnt^M n=4; wild type n=4; homozygous Mnt (Hom) n=2 (n=sample number analysed). (B) Histogram of Igf2 (3.8 kb transcript) relative expression levels obtained in A normalised against Gapdh for each class of intercross sample, error bars show the standard deviations. (C) Circulating Igf2 serum (ng/ml) levels in neonates (P1) following paternal (n=12) and maternal (n=17) transmission of Mnt, and their corresponding wild-type littermates (n=9 and n=11, respectively). Error bars show the standard deviations.

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