Fig. 4. Expression and methylation analysis with maternal transmission of Mnt. (A) Northern analysis of Igf2 and H19 in neonatal (P1) in Mnt^M and the corresponding wild-type littermates. (B) Representation of the Igf2 expression levels normalised against Gapdh (in tissues in which Igf2 expression was detectable after paternal transmission). Error bars show the standard deviations when multiple samples have been analysed. Tongue wild type n=1, Mnt^M n=1; lung wild type n=4, Mnt^M n=3; liver wild type n=5, Mnt^M n=3; intestine wild type n=4, Mnt^M n=4; muscle wild type n=1, Mnt^M n=1. (C) Methylation analysis of H19 DMR in homozygous Mnt foetuses (E17) using a 3.8 kb SacI probe, hybridised to SacI- and AatII-(methylation sensitive) digested genomic DNA (Tremblay et al., 1995). Note the absence of the unmethylated allele in the homozygous Mnt sample, showing methylation of the maternal Mnt allele, while in the wild type the maternal allele is unmethylated. (D) Allele specific expression analysis of Igf2 in Mnt^M neonatal (P1) tissues (MntxSD7) and the corresponding wild-type littermates (F₁xSD7) by RT-PCR (Dean et al., 1998) (three individual samples analysed). The 602 bp band corresponds to the maternal allele, while the 473 bp corresponds to the paternal SD7 allele.

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