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Fig. 10. Embryos were injected at the one-cell stage with 0.5 ng Xanx-4 mRNA or 10 ng Xanx-4 MO and cultured to stage 25. Groups of five embryos were then subjected to RT-PCR using primers designed against a range of pronephric molecular markers. No effect on expression of any of the markers was observed. EF1
was used as a loading control.
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