Fig. 2. F1 heretozygotes of high-copy number founders were embryonic lethal. (A) Lateral view of E9.5 nontransgenic and transgenic embryos from the H1 founder. All E9.5 transgenic embryos were markedly growth-retarded with severe cardiac defect with a dilated pericardium. E9.5 transgenic embryos from the H1 and H2 founders were morphologically undistinguishable. A, atrium; V, ventricle; PS, pericardial sac. (B) Genotypic and phenotypic analysis of F1 embryos from the H1 founder. PCR screening was performed with DNA extracted from the yolk sac of the embryos at E8.5, E9.5, E10.5, E11.5 and from the tail of weaned mice. Some of the E10.5 and all of the E11.5 transgenic embryos were dead and in the process of being resorbed (dead). (C) Genotypic and phenotypic analysis of F1 embryos from the H2 founder.
