Fig. 5. Increased cardiac expression of Rho GDI
inhibited the activity of Rho family members. (A) RhoA, Rac1, Cdc42 and RhoGDI were expressed in the early developing hearts. Western blot analysis of RhoA, Rac1, Cdc42 and Rho GDI was performed with protein samples prepared from a pool of 10 hearts from E9.5 or E11.5 embryos. (B) Membrane translocation of RhoA, Rac1 and Cdc42 was inhibited in the transgenic hearts. Western blot analysis of RhoA, Rac1 and Cdc42 was performed with the cytosolic and membrane fractions of cardiac and skeletal proteins extracted from heterozygotes and nontransgenic littermates of the M2 line.
