Fig. 7. Activated Erk1/2 and p90Rsk2 associate with meiotic chromosomes. Chromatin and/or chromosomes were obtained by hypotonic treatment of spermatocytes and were attached directly on a microscope slide for immunofluorescence analysis as described in the legend to Fig. 5. (A) Chromatin was stained with anti-p90Rsk2 antibody (right panels) or Hoechst dye (left panels). (B) Chromatin was stained with anti-phosphoErk1/2 antibody (right panels) or Hoechst (left panels). Both p90Rsk2 and activated Erks colocalize with meiotic chromosomes.
