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Fig. 4. GAL4-EcR and GAL4-USP are widely activated by 20E in cultured larval organs. Larval organs dissected from heat treated hs-GAL4-EcR; UAS-nlacZ (GAL4-EcR) or hs-GAL4-USP; UAS-nlacZ (GAL4-USP) third instar larvae 18-8 hours before puparium formation were cultured with 5x10–6 M 20E and processed for histochemical staining. GAL4-EcR and GAL4-USP are not activated in culture in the absence of 20E (data not shown). Both GAL4-EcR (A-D) and GAL4-USP (E-H) are widely activated by 20E in many larval tissues, including the midgut, hindgut, muscles and fat body.





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