Fig. 5. GAL4-EcR and GAL4-USP can be activated by 20E in the ring gland and imaginal discs. Larval organs dissected from heat treated hs-GAL4-EcR; UAS-nlacZ (GAL4-EcR) or hs-GAL4-USP; UAS-nlacZ (GAL4-USP) third instar larvae 18-8 hours before puparium formation were cultured with 5x10–6 M 20E and processed for histochemical staining. Both GAL4-EcR and GAL4-USP are activated by 20E in the ring gland (B,C) and imaginal discs (E, wing imaginal disc is shown; F, leg imaginal disc is shown) but not in the absence of hormone (A, ring gland; D, both wing and leg imaginal discs are shown).
