Fig. 6. Identification of PAG-3-staining ventral cord neurons in adult animals. Otherwise wild-type adult C. elegans carrying an integrated P_unc-4lacZ transgene were co-stained with PAG-3 antiserum and monoclonal antibody directed against ß-galactosidase. DAPI was used to identify nuclei. (A-D) The posterior ventral cord; anterior is leftwards and ventral is downwards. VA11 and VA12 are marked with open triangles. (E-H) The retrovesicular ganglion and anterior ventral cord; ventral view, anterior is leftwards. AVFR and AVFL are marked with filled triangles. (A) DAPI, (B) ß-galactosidase expression in VA11 and VA12 (Miller and Niemeyer, 1995). (C) PAG-3 staining. (D) Merged image. (E) DAPI staining of nuclei in the retrovesicular ganglion. (F) ß-galactosidase expression in five cells in the retrovesicular ganglion. P_unc-4lacZ is expressed in the AVF interneurons, the VA motoneurons, and additional cells in the retrovesicular ganglion (Miller and Niemeyer, 1995). (G) PAG-3 staining. By several criteria (see text), the two anterior-most PAG-3 expressing cells in the retrovesicular ganglion are the right and left AVF interneurons. The two more posterior PAG-3-positive cells in the retrovesicular ganglion are the embryonically generated right and left RIG interneurons; they are marked with asterisks. (H) Merged image.

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