Fig. 5. Transgenic epidermal differentiation markers are perturbed. Newborn transgenic and wild-type epidermis was processed for immunofluorescence to evaluate epidermal differentiation markers. K5 expression was not altered in the transgenic epidermis, however, K1 expression was increased indicating a perturbation in the differentiation program of the transgenic epidermis. In addition, K6, which is not normally expressed in the epidermis, shows some patchy expression in the transgenic epidermis; while normal hair follicle (HF) distribution was not altered. In addition, filaggrin, loricrin and transglutaminase-3 were also assessed. There was aberrance in the expression of these markers in the transgenic epidermis in that they were much more diffuse and expressed in a much broader zone, especially in the case of filaggrin.
