Fig. 6. The overexpression of claudin 6 causes an overall disturbance in the expression of other markers. (A) Western blot analysis revealed the enhanced processing of profilaggrin. The transgenic samples revealed a ‘bottom heavy’ expression of the smaller sized processed filaggrin while wild-type samples were ‘top heavy’ indicating a greater abundance of unprocessed profilaggrin in the wild-type newborn epidermis. In fact, processed filaggrin was increased 
13-fold. In addition, the expression of loricrin, transglutaminase-3 and involucrin was increased 1.5 fold in the transgenic samples. (B) RT-PCR analysis indicated no significant differences with filaggrin, loricrin, transglutaminase-3 and involucrin in the transgenic epidermis versus wild-type epidermis. (C) RT-PCR analysis further indicated that repetin, members of the SPRR family and Klf4 (genes involved in CE formation) were altered in transgenic epidermis. Repetin, SPRR1A, 2A and Klf4 were downregulated in the transgenic samples while the expression of SPRR2D and 2G was increased. SPRR1B, 2B, 2C and 3 remained unchanged.
