Fig. 1. NotchIC induces ectopic activation of sim and m8. Cellularizing embryos express a stripe2-NotchIC transgene and are oriented with dorsal upwards and anterior towards the left. Transgenic embryos were hybridized with digoxigenin-labeled Notch (A), m8 (B) and sim (C) antisense RNA probes, and stained to visualize the indicated gene expression patterns. (A) The Notch probe detects an ectopic stripe of NotchIC expression. General staining of the wild-type Notch RNA is detected throughout the basal cytoplasm. (B) The m8 gene exhibits endogenous lateral lines of expression in the mesectoderm, as well as an ectopic stripe. (C) Ectopic sim expression is restricted to a pyramid pattern in the neurogenic ectoderm.
