Fig. 5. The stripe2-snail transgene induces complex changes in the m8 and sim expression patterns. Wild-type (A-F) and Tollrm9/Tollrm10 (G-L) were stained with m8 (A-C;G-I) or sim (D-F;J-L) hybridization probes. (A-C) Lateral views of wild-type embryos that either lack (A) or carry (B,C) the stripe2-snail transgene. In early embryos (just after cellularization), the transgene creates a gap in the normal lateral lines within the presumptive mesectoderm (arrowhead, B; compare with A). In older embryos (gastrulation) there is both a gap in the pattern and ectopic staining in lateral regions (arrowhead, C). (D-F). Lateral views of wild-type embryos that either lack (D) or carry (E,F) the stripe2-snail transgene. There is a gap in the pattern in early embryos (arrowhead in E; compare with D), but the top of the gap is filled to produce a continuous bump of staining in older embryos (arrowhead, F). The asterisk indicates a gap in the sim pattern that is due to normal discontinuities in the initial sim pattern, not to the stripe2-snail transgene. (G-I) Mutant embryos obtained from Tollrm9/Tollrm10 females. There is no m8 expression in middle body regions of mutant embryos (G), although there is expression at the posterior pole. Mutants that express the stripe2-snail transgene exhibit broad stripes of m8 staining (H). Mutants that express the stripe2-snail/hairy transgene also exhibit ectopic m8 staining (I). (J-L) Mutant embryos derived from Tollrm9/Tollrm10 embryos. sim expression is restricted to the termini of mutant embryos lacking the stripe2-snail transgene (J). By contrast, patchy stripes of sim expression are observed both in mutants that contain the stripe2-snail transgene (K) and the stripe2-snail/hairy transgene (L).
