Fig. 5. Expression of liver-enriched transcription factors in control (+/+ and +/–) and in Hnf6–/– mice. The expression levels were assessed by semi-quantitative RT-PCR on liver RNA. The data are representative of three independent experiments. (A) At E14.5, the expression levels of Hnf3ß, Hnf3
and Hnf4
are not affected by inactivation of the Hnf6 gene. The expression levels of Hnf1 and Hnf3 are slightly increased in Hnf6–/– fetuses, whereas the two splicing isoforms of Hnf1ß (Hnf1ß-A and Hnf1ß-B) are strongly downregulated. At P3, the expression levels of Hnf3ß, Hnf3 and Hnf4 are not affected, the expression of Hnf1ß has normalized and that of Hnf1 and Hnf3 is still upregulated. (B) At E12.5, the expression of the two splicing isoforms of Hnf1ß is strongly downregulated in the Hnf6–/– livers, as assessed with Tbp as a reference.
