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Fig. 5. Lack of arteries in mutant portal tracts. Immunostaining for {alpha} smooth muscle actin (SMA) on control (CO) and mutant (M) liver sections, isolated two months after birth. (A,C) Control livers show SMA-positive arteries (arrowheads) located around portal veins. Portal veins (pv) are also stained with the antibody. (B,D) In mutant livers almost all structures stained are veins. Only few large arteries are present. Scale bars: 100 µm.





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