Fig. 7. (A,B) HNF1ß is required for the general lipid homeostasis. Biochemical analysis of cholesterol (A) and triglycerides (B) levels in mutant (black bars) and control (gray bars) mice at the age of 1 week (n=8), 2 weeks (n=6) and 8 weeks (n=6). All differences between mutant and controls were significant (P<0.05). There is no significant difference between 2- and 8-week-old mutants. Triglyceride level for 1-week-old mutant is abnormally elevated despite the lack of comparison with the control. Standard deviation for each sample is featured as error bars. (C,D) HNF1ß is required for the expression of the Oatp1 transporter and the fatty acid dehydrogenase VLCAD. (C) Semi-quantitative RT-PCR analysis of liver organic anion transporters expression (Slc21a1/Oatp1; Slc21a5/Oatp2; Slc21a6/Oatp4). Genotypes are indicated on top. HPRT primers were used as a normalizing standard for the RT samples (HPRT) and as a reverse transcription control (HPRT –RT). Gene expression was measured in six mice for each genotype. (D) Northern blot analysis of very long chain acetylCoA dehydrogenase (VLCAD) mRNA levels. HPRT cDNA probe is used as a normalizing standard. Gene expression was measured in four mice for each genotype. Ratios between mutants and controls are shown after HPRT normalization (M/CO).
