Fig. 1. yellow and ebony are required for proper pigment patterning in D. melanogaster. (A) Wild type D. melanogaster females produce a stripe of dark pigment (arrowhead) near the posterior edge of abdominal segments A2-A6. (B) In yellow null mutants, black melanin is lost within the stripe, leaving a brown pigment, and cuticle anterior to the stripe has a tan appearance relative to wild type. (C) ebony1 mutants retain a distinct pigment stripe and the cuticle anterior to the stripe is much darker than wild type. (D) yellow; ebony1 double mutants have brown pigment throughout the abdomen and the stripe is no longer distinct. Similar changes in pigmentation are observed in other structures. Wild-type flies are a uniform color in the thorax (E) and wings (I). In yellow mutants, these structures become tan (F,J). In ebony1 mutants, the thorax (G) and wings (K) are more darkly pigmented, and new pigment patterns are visible (arrowheads). yellow; ebony double mutants also show these patterns, but the black pigment is absent, and they consist of two shades of brown pigment (H,L, arrowheads). In all panels, anterior is upwards and dorsal cuticle is shown. Abdominal segments A3-A5 are shown in A-D.
