Fig. 2. Antibodies specific for the Drosophila Yellow and Ebony proteins. Western blots of 60-72 hour pupal protein extracts. Antibodies to the Yellow protein produced in rabbits (A) and rats (B) recognize a 60 kDa protein in protein extracts from animals with a wild-type y gene (Canton^S, e^AFA), but not from y mutants (y¹⁷⁰, yw, yw^ac). Both antibodies also recognize a similar size protein in D. biarmipes. (C) Antibodies raised in rabbits against the Ebony protein recognize a 94 kDa protein (arrowheads) in wild-type animals (Canton^S), but not e mutants (e¹, e^AFA). Several smaller proteins in both wild-type and e mutant flies are also detected (data not shown). A protein of approximately 94 kDa is recognized in extracts from D. biarmipes. A strong band just below the presumptive Ebony band also appears in both wild-type and e mutant extracts. (A-C) The panel below each blot indicates relative protein loading.

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