Fig. 6. Complementary patterns of Yellow and Ebony expression correlate with the formation of a novel, male-specific, black melanin pattern in D. biarmipes wings. (A) A spot of black melanin (arrow) is present in the wings of D. biarmipes males. (B,D) Yellow protein (purple) is expressed at higher levels in the cells that produce this spot (arrow) than in the surrounding wing. (C,D) Ebony expression (green) is lower in these cells (arrow) than in the rest of the wing. (E-H) The boundaries between expression levels of Yellow and Ebony coincide (F-H, arrows) and correlate with the boundary of pigment in adult wings (E, arrow). (I) D. biarmipes females, typically do not produce a pigment spot in the wing, and both Yellow (J,L) and Ebony (K,L) proteins are uniform throughout the wing in most females. In some adult females, a small, faint pigment spot is observed (data not shown). Consistent with this phenotypic variation, a few cells expressing higher levels of Yellow are present in a small proportion of the female pupal wings (data not shown).
