
Fig. 7. Dosage analysis of Xcl1. (A) Large, colored kernel with hyperploid endosperm and hypoploid embryo. (B) Small, yellow kernel with hypoploid endosperm and hyperploid embryo with a colored scutellum (arrow). (C) TB-10L kernel hypoploid for xcl1 has one layer of aleurone. (D) TB-10L kernel hyperploid for xcl1 has regions with more than one layer of aleurone. (E-I) Free-hand transverse sections of leaves. (E) The hypoploid (Xcl1/0) leaf is thinner than wild-type and has no extra cell layers beneath the epidermis. (F) A wild-type (xcl1/xcl1) leaf. (G) Diploid (Xcl1/xcl1) leaf has infrequent patches of extra cell layers. (H) Hyperploid (Xcl1/xcl1/xcl1) leaf has a more severe extra cell layer phenotype than the diploid. (I) Homozygous diploid (Xcl1/Xcl1) leaf has the most severe extra cell phenotype. Scale bars A,B, 2 mm; C,I, 100 µm.