Fig. 8. Comparative analysis of mineralization and expression of osteoblastic markers in long bones and calvaria. At E16.5, there are dramatic differences in the mineralization of wild-type (A, Alizarin Red; C, von Kossa) and VEGF^120/120 (B, Alizarin Red; D, von Kossa) tibias (A,B) and radii (C,D). Most of the mineralization that is observed in the tibia and radius of VEGF^120/120 mice is not associated with osteoblastic activity, but is hypertrophic zone mineralization (hypertrophic zone identified by bold line, A and B). At E16.5, expression of Col1a1 in wild-type tibia (E) is much more extensive than in VEGF^120/120 tibia (F). At E16.5, expression of osteocalcin in wild type (G) is much stronger than that in VEGF^120/120 radius and ulna (H) (arrowheads identify regions of expression). At both E15.5 and E16.5, reduced mineralization is observed in VEGF^120/120 mouse calvaria (J,L,N) when compared with wild-type calvaria (I,K,M). At E16.5, expression of Col1a1 is significantly stronger in the wild-type calvarium (O) than in the VEGF^120/120 calvarium (P).

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