Fig. 3. Pelle is autophosphorylated when co-transfected with constitutive active Toll10b. (A) The indicated amounts of DNA were transfected into SL2 cells, and then analyzed as Fig. 2. Lane 1, 0.3 µg of Toll^10b; lane 2, 0.1 µg of Pelle; lane 3, 0.3 µg Toll^10b plus 0.1 µg Pelle; lane 4, 0.1 µg PelleK240R; and lane 5, 0.3 µg Toll^10b plus 0.1 µg of PelleK240R. Western blots of transfected cell lysates: top, anti-Pelle blot; bottom, anti-Dorsal blot. (B) CAT assays for the same transfections were performed as in Fig. 2.

Return to article