Fig. 7. KNAT2 GUS reporter gene expression from the gene trap insertion GT7953. GUS reporter gene expression is observed in the embryonic (A) and seedling (B) SAM. In the inflorescence, GUS reporter gene expression is detected in the apex and young flowers but not in more mature flowers (C). Viewed under DIC microscopy (D), GUS activity (pink) is found in the inflorescence and floral meristems and in all organs of young flowers but in more mature flowers is confined to the carpels (inset). In as1 (E) and as2 (F), GUS reporter gene expression expands from the SAM into the base of the leaves. RT-PCR amplification using gene-specific primers and hybridization of products with gene-specific probes (G) show that KNAT2 transcripts are detected in wild type but not in plants homozygous for the gene trap insertion (top panel). RBC transcripts were ampilfied as a control (bottom panel).
