
Fig. 5. (A) Effect of poxn overexpression induced by heat shock for 30 minutes, 37°C at 8 hours APF. In the leg, MBs are transformed into ChBs and bracts are missing. (B) Overexpression of poxn and Raf* yields an additive phenotype, with trichomes transformed into bracts and MBs into ChBs. (C-E) poxn expression detected by poxn RNA in situ hybridisation in early pupal leg (C) and wing (E) of wild-type, and leg of In(1)sc101 (D). (F) hsp70-GAL4/UAS-sSpi leg. All the trichomes are transformed into bracts. (G,H) rho1-lacZ expression in wild-type and In(1)sc101 legs, respectively. (I) A rho1de11 clone marked with f is outlined. Note the absence of bracts within the clone (arrows) and the presence of bracts in the wild-type bristles that surround the clone (arrowheads). (J) UAS-rho1 and UAS-S overexpression by hsp70-GAL4. Note the presence of three bracts in some bristles (arrows).