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Fig. 2. Cell sorting of different CD34 and CD41 populations from EB6 cells. Double staining was performed with a FITC-conjugated anti-CD41 MoAb and a biotinylated anti-CD34 MoAb, using a streptavidin-R-PE as the secondary reagent. Four cell subsets (CD34+CD41+, CD34CD41+, CD34+CD41 and CD34CD41) were sorted by flow cytometry in R1, R2, R3 and R4, respectively. The percentages are shown for one representative experiment (n=4).
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