Fig. 11. Molecular and cellular effects of RUNX1-CBF2T1 expression at 48 hpf. Whole-mount in situ hybridization for hbbe3 (globin) (A,B), flk-1 (E,F) and myb (I,J) expression. Lateral views, anterior towards the left. Cells aspirated from the ICM region (C,D) and circulation (G,H,K,L). Controls are indicated. (A,B) hbbe3 (globin) expression is reduced in the circulation (arrowhead) and tail (arrow) compared with control. (E,F) flk-1 expression in the tail (arrows) is abnormal. (I,J) myb expression is markedly reduced in the dorsal aorta (arrows). (C) Immature, blast-like cells accumulated in the ICM. (D) Cluster of atypical early cells (arrow). (G) Nuclear bridge (arrowhead), doughnut-shape nucleus (arrow). (H) Binuclear cell (arrowhead). (K) Cleaved nuclei (black arrowheads), mitotic figure (arrow) and a megaloblast (purple arrowhead). Scale bars: 
10 µm.
