Fig. 3. Characterization of runx1 expression. (A-E) Two-color in situ hybridization using runx1 (purple; NBT/BCIP) and scl (red; Fast Red) riboprobes (purple and red arrowheads, respectively). (C,E) Fluorescence of scl signal using rhodamine filter. (F-I) Co-labeling of embryos hybridized with runx1 riboprobe (purple) with anti-HNK-1 (brown) (purple and brown arrows respectively). (A) Dorsal view of whole embryo. (B,C) Higher magnifications of an area boxed in A. (D,E) Lateral view of posterior portion of embryo, anterior towards the left. (F-I) Dorsal views of mid-trunk embryo regions, anterior towards the left. (G,I) Higher magnifications of areas boxed in F,H, respectively. Stages in hpf are indicated. (A) runx1 and scl overlap in the LPM (arrowheads). (B,C) Overlap of runx1 and scl in individual cells (arrowheads). (D,E) runx1 expression is weaker than scl in the posterior ICM. (F,G) Rohon-Beard cells with no runx1 expression (brown arrowheads), and a cell with dual expression (purple arrowhead). (H,I) Putative cranial nerve VIII nuclei with overlapping runx1 and HNK-1 expression (purple arrowheads). Otic vesicle (ov) is indicated.
