Fig. 5. Phenotypic effects of runx1-MO injections. (A-H) Morphological changes at 24 hpf (A-C), 48 hpf (D-H) and circulation defect visualized by microangiography at 52 hpf (I,J). All are lateral views of whole embryos (A-E), posterior halves of embryos (F-H) and mid-trunk regions (I,J), anterior towards the left. Control embryos are indicated. (A,B) Blood cells accumulated in the anterior (arrows) and posterior (arrowheads) ICM in comparison with control in C. (D) Lack of normal circulation. Blood cells accumulated in the aorta (region encompassed by two black arrows). Empty heart and edematous vitelline vessels (red arrow). Underdeveloped head (red arrowhead). Otic vesicle containing three otoliths (black arrowhead). (E) Normal circulating blood cells (arrow). (F,G) Collections of blood cells in ventral tail ICM (arrowheads). (H) Normal tail circulation with caudal artery (arrowhead) and vein (arrow). (I) Interrupted aortic blood flow (red arrow) with lack of flow in cardinal vein and intersegmental vessels. (J) Normal circulation; dorsal aorta (red arrow), posterior cardinal vein (green arrow) and intersegmental vessel (red arrowhead).
