Fig. 9. Effects of RUNX1-CBF2T1 expression in zebrafish embryos. (A) Western analysis of Jurkat cell lysate (lane 1) and extracts from 18 hpf embryos injected with pCS2cmv-RUNX1-CBF2T1 (lane 2), pCS2 vector alone (lane 3) and uninjected embryos (lane 4). (B) Tabulated summary of phenotypes generated by transient expression of RUNX1-CBF2T1 following injection of construct at two doses, compared with pCS2 vector alone and uninjected embryos. (C-N) Morphological changes at 24 hpf (C,D) and 48 hpf (E-N). (C-J) Whole embryos and (K-N) head regions; lateral views except L, which is ventral; anterior towards the left for all. Controls are indicated. (C,D) Blood accumulated in the ICM region compared with normal (arrowheads). (E,F) Embryos with no circulation and blood accumulated in the proximal aorta (arrow) and ICM (arrowhead). (G,H) Aberrant circulation with blood pooling in the ICM (arrows) and associated CNS bleed (arrowhead). (I) Normal circulation with CNS hemorrhage (arrowhead). (K-M) Arrowheads indicate intraventricular, intracerebral and pericardial hemorrhages respectively.
