Fig. 5. Seam cell defects of plx-1(nc37) hermaphrodites. Lateral views with anterior towards the left. All animals contain jcIs1; him-5. (A,C,E) DIC images of adult alae (arrowheads) in a control (A) and plx-1 mutant (C,E) hermaphrodites. In plx-1 animals, alae were discontinuous (arrows, C) or bifurcated (E). (B,D,F) Fluorescent images of the jam-1::gfp transgene expression in a control animal (B) and plx-1 mutant animals (D,F) shown in A,C,E. (D) In a plx-1 animal, the seam cell boundaries closed midway (arrows). The position of the gap without seam cells corresponds with that of the gap of the alae. (F) In a plx-1 animal, extra cell boundaries formed within a seam cell where the alae made bifurcations. (G,H) Fluorescent images of the jam-1::gfp transgene expression in a control (G) and a plx-1 L4 (H) hermaphrodite. In the plx-1 animal, the arrangement of seam cells is disrupted (arrowheads). Some seam cells were separated by gaps (asterisks). Scale bars: 10 µm.
