Fig. 1. Multiple sequence alignment of invertebrate semaphorins. Multiple sequence alignment of members of the class I semaphorin family using the ClustalX program. The alignment includes Tribolium semaphorin 1a (T-Sema-1, Accession Number L26080), Grasshopper semaphorin 1a (G-Sema-1, Accession Number L00709), Drosophila melanogaster semaphorin 1a and semaphorin 1b (D-Sema-1a and D-Sema-1b, Accession Numbers AAF52696 and AAF57816, respectively), C. elegans semaphorin 1a and semaphorin 1b (Ce-Sema-1a and Ce-Sema-1b, Accession Number Q17330 and Accession Number unpublished, respectively). Completely conserved residues are highlighted in black, similar residues in gray. C. elegans Ce-Sema-1a and Ce-Sema-1b predicted domains and motifs are as indicated: signal sequence for secretion (purple), semaphorin domain (yellow), Furin protease consensus cleavage site (green), Met related sequence (MRS) (blue), transmembrane domain (black) and a predicted PDZ binding domain (red).
