Fig. 5. Mutation of the LFY binding site in the AP3 promoter does not disrupt expression in planta. The 5D3::GUS construct containing site Im and site IIm mutations (5D3-ImIIm::GUS) confers GUS expression in the presumptive petal and stamen primordia in a wild-type background both at stage 3 (A) and at stage 8 (B), similar to that seen for the unmutated version of 5D3::GUS (Hill et al., 1998). 5D3-ImIIm::GUS also confers GUS activity in 35S::LFY, 35S::UFO seedlings (C). Expression of the D3-18 construct containing site Im and site IIm mutations (D3-18-ImIIm) in a wild-type background at stage 6 (D) and stage 10 (E). This pattern of GUS expression recapitulates that conferred by the unmutated D3-18::GUS construct (Hill et al., 1998). Furthermore, D3-18-ImIIm::GUS is expressed in seedlings containing the 35S::LFY, 35S::UFO transgenes (F).
