Fig. 2. Mos triggers GVBD independently of MAPK activation. Oocytes were incubated in U0126 1 hour before oligonucleotide injection, and progesterone was added 1 hour after microinjection. (A) Oocytes were microinjected with Mos antisense oligonucleotides in presence (AS Mos + U0126) or absence (AS Mos) of U0126, or were non-injected in the presence (U0126) or absence (control) of U0126. Homogenates from resting oocytes (stage VI) or oocytes stimulated for 20 hours by progesterone were analyzed by western blot with different antibodies as indicated. (B) Mos antisense-injected oocytes undergo GVBD with or without U0126. Oocytes treated as in A were scored as a function of time for GVBD (observed as appearance of a distinct white spot).
