Fig. 8. Myt1 is phosphorylated in vivo, although MAPK activation is inhibited. (A) Timecourse of Myt1 phosphorylation during maturation of oocytes incubated in U0126 1 hour before progesterone addition. (B) Timecourse of Myt1 phosphorylation during maturation of oocytes non-treated (Control) or incubated in U0126 and injected (AS Mos + U0126) or not (U0126) with Mos antisense oligonucleotides before progesterone addition. Groups of five oocytes were collected per point. The equivalent of one oocyte was loaded per lane and was analyzed by immunoblot with the indicated antibodies. Control M: non-treated mature oocyte.
