Fig. 5. Transgenic rescue of vab-1 ptp-3 synthetic lethality by PTP-3B. Embryonic lethality was quantitated in transgenic strains as described in Materials and Methods. All strains are homozygous for vab-1(e2) and ptp-3(op147). (A) ptp-3(+) transgenes (4 lines) contain the cosmid F38A3; PTP-3B::GFP transgenes (3 lines) contain the PTP-3B::GFP transgene injected at low concentration (see Materials and Methods). The vab-1 ptp-3 synthetic lethality is suppressed in lines containing these transgenes, relative to control extrachromosomal arrays containing the pRF4 marker plasmid (juEx195). Data for vab-1(e2) (George et al., 1998) are shown for comparison. (B) Suppression of vab-1 ptp-3 synthetic lethality by neuronally expressed PTP-3B (juEx377-379) but not by epidermally expressed PTP-3B (juEx422-424). Only arrays juEx378 and juEx379 caused significant reduction in lethality (Student’s t-test, P<0.01).
