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Fig. 6. XHes6 and XHes6DBM increase myotome size. Embryos were injected with 2 ng of XHes6 (A,D,G), XHes6DBM (B,E,H) or XHes6{Delta}WRPW (C,F,I) along with ß-gal (light blue, injected side to left) and analysed at the neural plate stage for NßTub (A-C), MyoD (D-F) or {alpha}-sarcomeric actin (MA) (G-I) expression by whole-mount in situ hybridisation (purple). Overexpression of all three constructs upregulated NßTub expression (A-C), while only XHes6 (D,G) and XHes6DBM (E,H) upregulated the muscle markers MyoD and MA.





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