Fig. 1. Wild-type pipe-lacZ expression is dynamic during oogenesis. (A-D) Confocal projections of egg chambers from females homozygous for the pipe-lacZ reporter construct. lacZ expression was detected using an anti-ß-gal antibody. Lateral views are shown, except where noted, with anterior to the left and dorsal at top. (A) At stage 8, pipe-lacZ is expressed in the anterior follicle cells that stretch to compensate for posterior migration (small white arrow) and in a sagittal ring (bracket) at the anterior margin of the posteriorly migrating group. Posterior follicle cells, and some posterior ventral follicle cells, express pipe-lacZ, with a gap in ventral expression about halfway across the columnar epithelium (arrowhead). (B) Dorsolateral view. As posterior follicle cell migration is completed at the end of stage 9, stretch cell and sagittal ring expression are maintained and ventral expression becomes contiguous. (C) Ventrolateral view. During stage 10A, the sagittal ring pattern gradually disappears, beginning dorsally and proceeding ventrally, such that expression protrudes dorsally from the anterior end of the ventral expression domain (arrowhead). (D) By stage 10B, dorsal sagittal ring expression has completely disappeared, while expression remains in the ventral, stretch (not shown), and posterior follicle cells. (E) Schematic diagram depicting wild-type pipe-lacZ expression in stretch (blue) and columnar (purple) follicle cells over time.
