Fig. 4. Dorsal appendage phenotypes resulting from Ras follicle cell clones. (A) The ‘extra nub’ phenotype: eggshell has an extra nub of chorion (arrowhead) in addition to two wild-type dorsal appendages. (B) The ‘nub’ phenotype: a nub of chorion material (white arrowhead) replaces one of the dorsal appendages. The other phenotypically wild-type appendage (a) is out of the plane of focus. The appendage of a neighboring egg can also be seen (black arrowhead). (C) The ‘two nubs’ phenotype: nubs replace both appendages (arrowheads). (D) Some chorion nubs are shaped like a teardrop (arrowhead), with a bulbous appendage base tapering to an anterior point. The other phenotypically wild-type appendage is out of the plane of focus (a). (E) Drawings, with corresponding frequencies, of the various chorion phenotypes seen in late stage Ras mosaic egg chambers. Frequencies are calculated from the number of eggs displaying a particular phenotype divided by the total number of eggs with chorion defects (n=177). Phenotypes labeled with (*) were selected for embryonic analysis.
