Fig. 6. Ras mutant cells within the dorsal appendage primordia fail to undergo a reduction in apical diameter. Confocal projections of apicolateral anti-E-cadherin (E-Cad) fluorescence in stage 12 egg chambers highlight the apical morphology of wild-type (A) and Ras mosaic (B) dorsal appendage primordia. DM, dorsal midline. (A) The bright apices of wild-type follicle cells undergoing dorsal appendage morphogenesis are uniformly small (arrowhead) unlike their neighbors, which have rather large apical surfaces. (B) Dorsolateral view. The left appendage primordium of a Ras mosaic egg chamber is disrupted. (C-E) Higher magnification views of the disrupted appendage primordium, boxed in B, reveal that Ras mutant cells – those lacking the myc epitope (C) – exhibit large apical footprints (D, arrowhead). Although the wild-type cells adjacent to the clone have small apical diameters, their overall organization is less uniform than expected.
