Fig. 2. Expression of the SP-C/G6en and SP-C/en transgenes during lung development. The SV40 poly(A)+ sequence was used as an in situ hybridization probe to detect the expression pattern of the SP-C/G6en (A-D,F,G) and SP-C/en (E) transgenes at the following time points during lung development: E17.5 (A,B), E19.5 (C-E), and P0 (F,G). There was no hybridization of the sense probe to the SV40 sequence (data not shown). Notice the restriction of transgene expression to the distal airway epithelium during lung development as has been reported previously with the human SP-C promoter (Wert et al., 1993). Scale bar: 250 µm.
