Fig. 5. SP-C/G6en mice have increased glycogen content in the distal airway epithelium. PAS staining was performed on histological sections of E19.5 wild-type (A) and SP-C/G6en mice (B,C) with (C) or without (A,B) prior treatment with amylase. At E19.5, wild-type littermates contain little glycogen in the distal airway epithelium (A), while SP-C/G6en mice show a significant increase in glycogen as determined by PAS staining (B). Pre-treatment with amylase (which digests the glycogen and eliminates PAS staining) demonstrates the specificity of the PAS staining (C). Scale bar: 80 µm.
