Fig. 5. Mosaic analysis of hnt clones in the eye. (A) The results of a clonal analysis of mosaic ommatidia along the borders of 94 hnt patches are schematized. Each data set represents ommatidia with different phenotypic organization: I, wild type; II, chiral flip; III, 45°rotation; IV, 90° rotation; V, chiral flip and 45° rotation; VI, chiral flip and 90° rotation; VII, symmetrical; VIII, symmetrical and chiral flip. Black R cells are wild type with respect to hnt; white R cells are mutant for hnt. The number of ommatidia with that particular phenotype is shown in red. (B) Apical tangential section of a hnt^XO01 clone (mutant patch is to the left of the red line). The polarity of each ommatidium is labeled with a pink arrow as shown on the schematic in A. The yellow asterisk marks the hnt single mutant cell (R6) in a mosaic ommatidium that is an example of class V (i.e. misrotated by 45° and chirally flipped with respect to the adjacent wild-type ommatidia). (C) More basal tangential section of a hnt^XE81 clone. A class VII symmetrical ommatidium with a mutant R4 cell (asterisk) is labeled with a yellow arrow. The R8 projection (between R1 and R2) is marked with an orange arrowhead. A chirally flipped and misrotated (class V) ommatidium can be seen on the bottom left of the mutant patch. In this case, there is a mutant R3 cell (asterisk).

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