Fig. 1. JH causes re-expression of BR in Manduca pupal epidermis. (A) Northern analysis of 10 µg total RNA from pupal forewings for the first 10 days after pupal ecdysis, using the Manduca br probe. Either 10 µl cyclohexane (normal) or 10 µg of the JH analog pyriproxifen in 10 µl cyclohexane was applied topically at 19 hours before pupal ecdysis. The Ethidium-stained ribosomal RNA indicates equal loading. (B) BR protein in a JH-treated pupal abdomen 5 days after pupal ecdysis as detected by immunocytochemistry with the Manduca BR core antibody. The JH treatment was as in A. Note the rosettes (R) of cells forming the pock marks of the second pupal cuticle.
