Fig. 3. JH causes re-expression of mRNAs for BR and a pupal cuticle protein Edg78E and suppression of mRNA for the adult cuticle protein Acp65A in the Drosophila abdomen during adult development. Northern analysis of 10 µg total RNA from separated anterior (head and thorax) and posterior (abdomen) regions after topical application of (A) 0.2 µl acetone (control) or (B) 100 ng pyriproxifen (JH mimic) in 0.2 µl acetone to the white puparium, using the probes described in the Materials and Methods. Note the predominant br transcript re-expressed during 36-72 hours APF is that of the 4.4 kb Z1 isoform (arrow). The Ethidium-stained ribosomal RNA indicates equal loading.
